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大鼠色素上皮衍生因子酶免試劑盒,(PEDF)ELISA檢測(cè)試劑盒,英文名:PEDF ELISA Kit,英文縮寫:PEDF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:大鼠色素上皮衍生因子ELISA檢測(cè)試劑盒,PEDF 檢測(cè)試劑盒,PEDF 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。大鼠色素上皮衍生因子酶免試劑盒,(PEDF)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性 人免疫球蛋白G Fc段受體Ⅱ(FcγRⅡ/CD32)ELISA試劑盒 Human Receptor Ⅱ for the Fc region of immunoglobulin G,FcγRⅡ ELISA KitY3356996T/48T
人免疫球蛋白G Fc段受體Ⅰ(FcγRⅠ/CD64)ELISA試劑盒 Human ReceptorⅠfor the Fc region of immunoglobulin G,FcγRⅠ ELISA Kit Y3357096T/48T
人粒細(xì)胞趨化蛋白-2(GCP-2/CXCL6)ELISA試劑盒Human granulocyte chemotactic protein-2,GCP-2 ELISA Kit Y3357196T/48T
人ω干擾素(IFN-ω/IFNB)ELISA試劑盒Human Interferon ω,IFN-ω ELISA KitY3357296T/48T
人糖基化依賴的細(xì)胞黏附分子(GlyCAM-1)ELISA試劑盒 Human glycosylation-dependent cell adhesion molecule-1,GlyCAM-1 ELISA KitY3357396T/48T
人干擾素調(diào)節(jié)因子(IRF)ELISA試劑盒 Human interferon Regulatory Factor,IRF ELISA KitY3357496T/48T
人淋巴毒素β(LTB)ELISA試劑盒 Human lymphotoxinβ,LTB ELISA KitY3357596T/48T
人淋巴毒素α(LTA)ELISA試劑盒 Human lymphotoxinα,LTA ELISA KitY3357696T/48T
人CC趨化因子受體1(CCR1)ELISA試劑盒Human CC-chemokine receptor 1,CCR1 ELISA KitY3357796T/48T
人CX3C趨化因子受體1(CX3CR1)ELISA試劑盒Human CX3C-chemokine receptor 1,CX3CR1 ELISA KitY3357896T/48T
人肺部活化調(diào)節(jié)趨化因子(PARC/CCL18)ELISA試劑盒 Human pulmonary activation regulated chemokine,PARC ELISA KitY3357996T/48T
人黏膜地址素細(xì)胞黏附分子(MAdCAM-1)ELISA試劑盒Human mucosal addressin cell adhesion molecule-1,MAdCAM-1 ELISA KitY3358096T/48T
人可溶性CD38(sCD38)ELISA試劑盒Human Soluble Cluster of differentiation 38,sCD38 ELISA KitY3358196T/48T
人可溶性CD21(CR2/sCD21)ELISA試劑盒 Human Soluble Cluster of differentiation 21,sCD21 ELISA KitY3358296T/48T
人可溶性瘦素受體(sLR)ELISA試劑盒Human Leptin Soluble Receptor,sLR ELISA KitY3358396T/48T
人Toll樣受體9(TLR-9/CD289)ELISA試劑盒Human Toll-like receptor 9,TLR-9 ELISA KitY3358496T/48T
人單核細(xì)胞趨化蛋白4(MCP-4/CCL13)ELISA試劑盒 Human monocyte chemotactic protein 4,MCP-4 ELISA Kit Y3358596T/48T
人N鈣黏蛋白/神經(jīng)鈣黏蛋白(N-Cad)ELISA試劑盒 Human Neural-Cadherin, N-Cad ELISA KitY3358696T/48T
人肝素結(jié)合性表皮生長(zhǎng)因子(HB-EGF)ELISA試劑盒Human heparin-binding epidermal growth factor-like growth factor,HB-EGF ELISA KitY3358796T/48T
人紅細(xì)胞刺激因子(ESF)ELISA試劑盒 Human erythropoiesis stimulating factor,ESF ELISA KitY3358896T/48T
人腫瘤壞死因子相關(guān)激活誘導(dǎo)因子(TRANCE)ELISA試劑盒 Human TNF related activation induced cytokine,TRANCE ELISA KitY3358996T/48T
人上皮細(xì)胞粘附分子(Ep-CAM/CD362)ELISA試劑盒 Human Epithelial Cell Adhesion Molecule,Ep-CAM ELISA KitY3359096T/48T
人巨噬細(xì)胞替代激活相關(guān)化學(xué)因子1(AmAC-1)ELISA試劑盒Human Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA KitY3359196T/48T
人可溶性血管內(nèi)皮生長(zhǎng)因子受體2(VEGFR-2/sFLK-1)ELISA試劑盒Human soluble vascular endothelial growth factor receptor-2,VEGFR-2/sFLK-1 ELISA KitY3359296T/48T
人胸腺基質(zhì)淋巴細(xì)胞生成素(TSLP)ELISA試劑盒Human thymic stromal lymphopoietin,TSLP ELISA KitY3359396T/48T
人可溶性CD28(sCD28)ELISA試劑盒human soluble cluster of differentiation 28,sCD28 ELISA Kit Y3359496T/48T
人胸腺活化調(diào)節(jié)趨化因子(TARC/CCL17)ELISA試劑盒 Human thymus activation regulated chemokine,TARC ELISA KitY3359596T/48T
人神經(jīng)細(xì)胞粘附分子配體1(NCAM-L1/CD171)ELISA試劑盒 Human Neural cell adhesion molecule ligand 1,NCAM-L1 ELISA KitY3359696T/48T
人可溶性凋亡相關(guān)因子配體(sFASL)ELISA試劑盒Human soluble Factor-related Apoptosis ligand,sFASL/Apo-1 ELISA KitY3359796T/48T
人細(xì)胞凋亡抑制因子(IAP)ELISA試劑盒Human inhibitor of apoptosis,IAP ELISA KitY3359896T/48T
人γ干擾素誘導(dǎo)單核細(xì)胞因子(MIGF/CXCL9)ELISA試劑盒Human monocyte interferon gamma inducing factor,MIGF ELISA KitY3359996T/48T
人干擾素誘導(dǎo)T細(xì)胞趨化因子(ITAC/CXCL11)ELISA試劑盒Human Interferon inducible T-cell Chemoattractant,I-TAC ELISA KitY3360096T/48T
人CD14分子(CDl4)ELISA試劑盒Human cluster Of differentiation,CDl4 ELISA KitY3360196T/48T
人白細(xì)胞共同抗原(LCA/CD45)ELISA試劑盒Human leukocyte common antigen,LCA/CD45 ELISA KitY3360296T/48T
人CD4分子(CD4)ELISA試劑盒Human cluster Of differentiation,CD4 ELISA KitY3360396T/48T
人CXC趨化因子配體16(CXCL16)ELISA試劑盒Human CXC-chemokine ligand 16,CXCL16 ELISA KitY3360496T/48T
人CXC趨化因子受體3(CXCR3)ELISA試劑盒Human CXC-chemokine receptor 3,CXCR3 ELISA KitY3360596T/48T
人γ干擾素誘導(dǎo)蛋白16/p16(IFI16/p16)ELISA試劑盒Human interferon-inducible protein 16,IFI16/p16 ELISA KitY3360696T/48T
人基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒Human Stromal cell derived factor 1a,SDF-1a ELISA KitY3360796T/48T
人可溶性CD86(B7-2/sCD86)ELISA試劑盒Human soluble Cluster of differentiation 86,sCD86 ELISA KitY3360896T/48T
人巨噬細(xì)胞移動(dòng)抑制因子(MIF)ELISA試劑盒Human Macrophage Migration Inhibitory Factor,MIF ELISA KitY3360996T/48T
人組織因子途徑抑制物(TFPI)ELISA試劑盒Human Tissue factor pathway inhibitor,TFPI ELISA KitY3361096T/48T
人干擾素誘導(dǎo)蛋白10(IP-10/CXCL10)ELISA試劑盒Human interferon-inducible protein 10,IP-10 ELISA KitY3361196T/48T
人巨噬細(xì)胞炎性蛋白5(MIP-5)ELISA試劑盒Human macrophage inflammatory protein 5,MIP-5 ELISA Kit Y3361296T/48T
人結(jié)締組織生長(zhǎng)因子(CTGF)ELISA試劑盒Human connective tissue growth factor,CTGF ELISA KitY3361396T/48T
人B細(xì)胞活化因子受體(BAFF-R)ELISA試劑盒 Human B cell activation factorr from the tumor necrosis factor family receptor,BAFF-R ELISA KitY3361496T/48T
人血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體3(VEGFR-3/Flt-4)ELISA試劑盒Human Vascuoar endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA Kit Y3361596T/48T
人血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體1(VEGFR-1/Flt1)ELISA試劑盒Human Vascuoar endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit Y3361696T/48T
人血管內(nèi)皮細(xì)胞生長(zhǎng)因子D(VEGF-D)ELISA試劑盒Human Vascular Endothelial cell Growth Factor D,VEGF-D ELISA KitY3361796T/48T
人血管內(nèi)皮細(xì)胞生長(zhǎng)因子C(VEGF-C)ELISA試劑盒Human Vascular Endothelial cell Growth Factor C,VEGF-C ELISA Kit Y3361896T/48T
各種類型ELISA測(cè)定時(shí),一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。