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小鼠垂體腺苷酸環(huán)化酶激活肽酶免試劑盒,(PACAP)ELISA檢測試劑盒,英文名:PACAP ELISA Kit,英文縮寫:PACAP ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠垂體腺苷酸環(huán)化酶激活肽ELISA檢測試劑盒,PACAP 檢測試劑盒,PACAP 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠垂體腺苷酸環(huán)化酶激活肽酶免試劑盒,(PACAP)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復性。
前蛋白轉(zhuǎn)化酶枯草溶菌素9(PCSK9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9)48T/96TY13483Homo sapiens (Human)
雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Doublecortin Like Kinase 1 (DCLK1)48T/96TY13484Homo sapiens (Human)
雙腎上腺皮質(zhì)激素樣激酶1(DCLK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Doublecortin Like Kinase 1 (DCLK1)48T/96TY13485Mus musculus (Mouse)
圍脂滴蛋白1(PLIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Perilipin 1 (PLIN1)48T/96TY13486Homo sapiens (Human)
跨膜絲氨酸蛋白酶4(TMPRSS4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transmembrane Protease, Serine 4 (TMPRSS4)48T/96TY13487Homo sapiens (Human)
波形蛋白(VIM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vimentin (VIM)48T/96TY13488Mus musculus (Mouse)
X-框結(jié)合蛋白1(XBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for X-Box Binding Protein 1 (XBP1)48T/96TY13489Mus musculus (Mouse)
醛脫氫酶1家族成員A1(ALDH1A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)48T/96TY13490Mus musculus (Mouse)
組氨酸豐富鈣結(jié)合蛋白(HRC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histidine Rich Calcium Binding Protein (HRC)48T/96TY13491Homo sapiens (Human)
MYC關(guān)聯(lián)因子X(MAX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for MYC Associated Factor X (MAX)48T/96TY13492Homo sapiens (Human)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY13493Equus caballus; Equine (Horse)
胸腺嘧啶核苷磷酸化酶(TP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymidine Phosphorylase (TP)48T/96TY13494Mus musculus (Mouse)
亮氨酸豐富α2-糖蛋白1(LRG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)48T/96TY13495Mus musculus (Mouse)
二氫嘧啶脫氫酶(DPYD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dihydropyrimidine Dehydrogenase (DPYD)48T/96TY13496Mus musculus (Mouse)
解聚蛋白(DSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Destrin (DSTN)48T/96TY13497Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶ω1(GSTω1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Omega 1 (GSTo1)48T/96TY13498Mus musculus (Mouse)
血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)48T/96TY13499Homo sapiens (Human)
組織蛋白酶Z(CTSZ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin Z (CTSZ)48T/96TY13500Homo sapiens (Human)
真核翻譯起始因子6(EIF6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eukaryotic Translation Initiation Factor 6 (EIF6)48T/96TY13501Homo sapiens (Human)
核糖核酸酶Ⅲ(RNASEN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribonuclease III, Nuclear (RNASEN)48T/96TY13502Homo sapiens (Human)
乙酰膽堿酯酶關(guān)聯(lián)蛋白(ACHAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acetylcholinesterase Associated Protein (ACHAP)48T/96TY13503Homo sapiens (Human)
活性依賴性神經(jīng)保護因子(ADNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activity Dependent Neuroprotector Protein (ADNP)48T/96TY13504Homo sapiens (Human)
腦富含膜附著信號蛋白1(BASP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Abundant, Membrane Attached Signal Protein 1 (BASP1)48T/96TY13505Homo sapiens (Human)
小泛素相關(guān)修飾蛋白2(SUMO2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Small Ubiquitin Related Modifier Protein 2 (SUMO2)48T/96TY13506Homo sapiens (Human)
Adropin蛋白(AD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adropin (AD)48T/96TY13507Homo sapiens (Human)
蛋白酶激活受體4(PAR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protease Activated Receptor 4 (PAR4)48T/96TY13508Homo sapiens (Human)
聚集蛋白聚糖(AGC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aggrecan (AGC)48T/96TY13509Rattus norvegicus (Rat)
成纖維細胞生長因子受體樣蛋白1(FGFRL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1)48T/96TY13510Homo sapiens (Human)
整合素α5(ITGα5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Alpha 5 (ITGa5)48T/96TY13511Homo sapiens (Human)
防御素α6(DEFα6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Defensin Alpha 6, Paneth Cell Specific (DEFa6)48T/96TY13512Homo sapiens (Human)
纖維蛋白肽B(FPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinopeptide B (FPB)48T/96TY13513Rattus norvegicus (Rat)
Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Runt Related Transcription Factor 2 (RUNX2)48T/96TY13514Mus musculus (Mouse)
激肽釋放酶7(KLK7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 7 (KLK7)48T/96TY13515Mus musculus (Mouse)
70kDa熱休克蛋白5(HSPA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)48T/96TY13516Mus musculus (Mouse)
受體活性修飾蛋白2(RAMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Receptor Activity Modifying Protein 2 (RAMP2)48T/96TY13517Homo sapiens (Human)
B類清道夫受體1(SCARB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Scavenger Receptor Class B Member 1 (SCARB1)48T/96TY13518Homo sapiens (Human)
ⅡA組磷脂酶A2(PLA2G2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Group IIA (PLA2G2A)48T/96TY13519Homo sapiens (Human)
糖原合酶激酶3β(GSK3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Synthase Kinase 3 Beta (GSK3b)48T/96TY13520Homo sapiens (Human)
核糖核酸酶L(RNASEL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribonuclease L (RNASEL)48T/96TY13521Homo sapiens (Human)
激活T-細胞核因子1(NFATC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Of Activated T-Cells, Cytoplasmic 1 (NFATC1)48T/96TY13522Mus musculus (Mouse)
Osterix蛋白(OSX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osterix (OSX)48T/96TY13523Mus musculus (Mouse)
C-型凝集素域家族2成員C(CLEC2C)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C-Type Lectin Domain Family 2, Member C (CLEC2C)48T/96TY13524Homo sapiens (Human)
溶血磷脂酸受體3(LPAR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysophosphatidic Acid Receptor 3 (LPAR3)48T/96TY13525Homo sapiens (Human)
溶血磷脂酸受體3(LPAR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysophosphatidic Acid Receptor 3 (LPAR3)48T/96TY13526Mus musculus (Mouse)
胸腺旁腺素(PTMS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Parathymosin (PTMS)48T/96TY13527Mus musculus (Mouse)
脂解激活脂蛋白受體(LSR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipolysis Stimulated Lipoprotein Receptor (LSR)48T/96TY13528Mus musculus (Mouse)
肌球蛋白重鏈4(MYH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 4, Skeletal Muscle (MYH4)48T/96TY13529Mus musculus (Mouse)
細胞程序性死亡蛋白1配體1(PDCD1LG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1)48T/96TY13530Mus musculus (Mouse)
同種異體移植炎癥因子1(AIF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Allograft Inflammatory Factor 1 (AIF1)48T/96TY13531Mus musculus (Mouse)
蛋白酶3(PR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proteinase 3 (PR3)48T/96TY13532Homo sapiens (Human)
黃嘌呤脫氫酶(XDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Xanthine Dehydrogenase (XDH)48T/96TY13533Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。