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小鼠白介素9酶免試劑盒,(IL-9)ELISA檢測試劑盒,英文名:IL-9 ELISA Kit,英文縮寫:IL-9 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠白介素9ELISA檢測試劑盒,IL-9 檢測試劑盒,IL-9 檢測試劑盒
ELISA實(shí)驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠白介素9酶免試劑盒,(IL-9)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
花生四烯酸-12-脂加氧酶(ALOX12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arachidonate-12-Lipoxygenase (ALOX12)48T/96TY11185Rattus norvegicus (Rat)
花生四烯酸-12-脂加氧酶(ALOX12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arachidonate-12-Lipoxygenase (ALOX12)48T/96TY11186Homo sapiens (Human)
花生四烯酸-12-脂加氧酶(ALOX12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arachidonate-12-Lipoxygenase (ALOX12)48T/96TY11187Mus musculus (Mouse)
抑制蛋白β1(ARRβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arrestin Beta 1 (ARRb1)48T/96TY11188Homo sapiens (Human)
抑制蛋白β2(ARRβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arrestin Beta 2 (ARRb2)48T/96TY11189Homo sapiens (Human)
防御素β1(DEFβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Defensin Beta 1 (DEFb1)48T/96TY11190Sus scrofa; Porcine (Pig)
胎球蛋白B(FETUB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fetuin B (FETUB)48T/96TY11191Rattus norvegicus (Rat)
載脂蛋白C2(APOC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein C2 (APOC2)48T/96TY11192Homo sapiens (Human)
載脂蛋白A5(APOA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A5 (APOA5)48T/96TY11193Homo sapiens (Human)
KSP鈣黏蛋白(CDH16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin 16 (CDH16)48T/96TY11194Homo sapiens (Human)
觸珠蛋白相關(guān)蛋白(HPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin Related Protein (HPR)48T/96TY11195Homo sapiens (Human)
肌微管鈣黏附蛋白(CDH15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin, Myotubule (CDH15)48T/96TY11196Homo sapiens (Human)
肝腸鈣黏蛋白(CDH17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin 17 (CDH17)48T/96TY11197Homo sapiens (Human)
白介素12B(IL12B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12B (IL12B)48T/96TY11198Chicken (Gallus)
白介素35(IL35)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 35 (IL35)48T/96TY11199Mus musculus (Mouse)
白介素35(IL35)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 35 (IL35)48T/96TY11200Homo sapiens (Human)
嗜鉻蛋白A(CHGA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chromogranin A (CHGA)48T/96TY11201Rattus norvegicus (Rat)
27kDa熱休克蛋白(HSPB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY11202Bos taurus; Bovine (Cattle)
17-α-羥化酶(S17αH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 17-Alpha-Hydroxylase (S17aH)48T/96TY11203Homo sapiens (Human)
膽固醇(CH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholesterol (CH)48T/96TY11204General
Ⅱ型前膠原(PCⅡ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen II (PCII)48T/96TY11205Homo sapiens (Human)
紅藻氨酸離子能谷氨酸受體2(GRIK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2)48T/96TY11206Homo sapiens (Human)
紅藻氨酸離子能谷氨酸受體2(GRIK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2)48T/96TY11207Rattus norvegicus (Rat)
叉頭框蛋白P3(FOXP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein P3 (FOXP3)48T/96TY11208Mus musculus (Mouse)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY11209Bos taurus; Bovine (Cattle)
白介素22(IL22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 22 (IL22)48T/96TY11210Homo sapiens (Human)
血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin I Converting Enzyme (ACE)48T/96TY11211Sus scrofa; Porcine (Pig)
血管內(nèi)皮生長因子受體2(VEGFR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)48T/96TY11212Rattus norvegicus (Rat)
熱休克蛋白β8(HSPβ8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 8 (HSPb8)48T/96TY11213Homo sapiens (Human)
肝脂酶(LIPC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Hepatic (LIPC)48T/96TY11214Sus scrofa; Porcine (Pig)
膽囊收縮素八肽(CCK8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholecystokinin 8, Octapeptide (CCK8)48T/96TY11215Rattus norvegicus (Rat)
膽囊收縮素八肽(CCK8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholecystokinin 8, Octapeptide (CCK8)48T/96TY11216Mus musculus (Mouse)
反三碘甲狀腺原氨酸(rT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Reverse Triiodothyronine (rT3)48T/96TY11217General
粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 5 Subtype AC (MUC5AC)48T/96TY11218Rattus norvegicus (Rat)
凝血因子Ⅷ(F8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor VIII (F8)48T/96TY11219Mus musculus (Mouse)
葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Transporter 4 (GLUT4)48T/96TY11220Homo sapiens (Human)
蛋白激酶Cζ(PKCζ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase C Zeta (PKCz)48T/96TY11221Mus musculus (Mouse)
蛋白酪氨酸磷酸酶受體O(PTPRO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type O (PTPRO)48T/96TY11222Mus musculus (Mouse)
線粒體乙醛脫氫酶(ALDM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM)48T/96TY11223Homo sapiens (Human)
酸性磷酸酶1(ACP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 1 (ACP1)48T/96TY11224Rattus norvegicus (Rat)
酸性磷酸酶1(ACP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 1 (ACP1)48T/96TY11225Mus musculus (Mouse)
角蛋白8(KRT8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 8 (KRT8)48T/96TY11226Homo sapiens (Human)
生長分化因子15(GDF15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 15 (GDF15)48T/96TY11227Homo sapiens (Human)
白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11228Chicken (Gallus)
生長分化因子15(GDF15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 15 (GDF15)48T/96TY11229Rattus norvegicus (Rat)
生長分化因子15(GDF15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 15 (GDF15)48T/96TY11230Mus musculus (Mouse)
金屬硫蛋白3(MT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metallothionein 3 (MT3)48T/96TY11231Rattus norvegicus (Rat)
纖維蛋白原降解產(chǎn)物(FDP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Degradation Product (FDP)48T/96TY11232Oryctolagus cuniculus (Rabbit)
骨鈣素(OC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteocalcin (OC)48T/96TY11233Oryctolagus cuniculus (Rabbit)
補(bǔ)體成分5(C5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 5 (C5)48T/96TY11234Rattus norvegicus (Rat)
S100鈣結(jié)合蛋白A7(S100A7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A7 (S100A7)48T/96TY11235Mus musculus (Mouse)
轉(zhuǎn)鐵蛋白(TRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transferrin (TRF)48T/96TY11236Bos taurus; Bovine (Cattle)
凝血因子ⅩⅢB肽(F13B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XIII B Polypeptide (F13B)48T/96TY11237Mus musculus (Mouse)
腦鈉素(BNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Natriuretic Peptide (BNP)48T/96TY11238Canis familiaris; Canine (Dog)
三磷酸肌醇(IP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inositol Triphosphate (IP3)48T/96TY11239General
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11240Chicken (Gallus)
免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A (IgA)48T/96TY11241Sus scrofa; Porcine (Pig)
磷脂酰肌醇特異性磷酯酶Cβ1(PLCβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase C Beta 1, Phosphoinositide Specific (PLCb1)48T/96TY11242Mus musculus (Mouse)
二?;视停―AG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diacylglycerol (DAG)48T/96TY11243General
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11244Bos taurus; Bovine (Cattle)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。