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小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒,英文名:IL-18 ELISA Kit,英文縮寫:IL-18 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠白介素18ELISA檢測試劑盒,IL-18 檢測試劑盒,IL-18 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY10535Sus scrofa; Porcine (Pig)
載脂蛋白A2(APOA2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein A2 (APOA2)48T/96TY10536Sus scrofa; Porcine (Pig)
脂聯(lián)素(ADP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adiponectin (ADP)48T/96TY10537Sus scrofa; Porcine (Pig)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY10538Sus scrofa; Porcine (Pig)
脂質(zhì)轉(zhuǎn)運(yùn)蛋白(CETP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cholesteryl Ester Transfer Protein (CETP)48T/96TY10539Oryctolagus cuniculus (Rabbit)
神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY10540Oryctolagus cuniculus (Rabbit)
轉(zhuǎn)化生長因子β1(TGFβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY10541Sus scrofa; Porcine (Pig)
丙酮酸脫氫酶磷酸酶(PDP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Pyruvate Dehydrogenase Phosphatase (PDP)48T/96TY10542Rattus norvegicus (Rat)
α2-纖溶酶抑制因子(α2PI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-2-Plasmin Inhibitor (a2PI)48T/96TY10543Oryctolagus cuniculus (Rabbit)
Ⅱ型前膠原羧基端原肽(PⅡCP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP)48T/96TY10544Rattus norvegicus (Rat)
60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY10545Chicken (Gallus)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY10546Rhesus monkey (Simian)
B-淋巴細(xì)胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY10547Bos taurus; Bovine (Cattle)
P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Selectin, Plaet (SELP)48T/96TY10548Oryctolagus cuniculus (Rabbit)
α-乳清蛋白(αLA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-Lactalbumin (aLA)48T/96TY10549Mus musculus (Mouse)
神經(jīng)介素S(NMS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuromedin S (NMS)48T/96TY10550Sus scrofa; Porcine (Pig)
促卵泡素(FSH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Follicle Stimulating Hormone (FSH)48T/96TY10551Homo sapiens (Human)
促腎上皮質(zhì)激素釋放激素(CRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Corticotropin Releasing Hormone (CRH)48T/96TY10552Homo sapiens (Human)
促腎上腺皮質(zhì)激素(ACTH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adrenocorticotropic Hormone (ACTH)48T/96TY10553Homo sapiens (Human)
促腎上腺皮質(zhì)激素(ACTH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adrenocorticotropic Hormone (ACTH)48T/96TY10554Mus musculus (Mouse)
促腎上腺皮質(zhì)激素(ACTH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adrenocorticotropic Hormone (ACTH)48T/96TY10555Rattus norvegicus (Rat)
促甲狀腺素釋放激素(TRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thyrotropin Releasing Hormone (TRH)48T/96TY10556Sus scrofa; Porcine (Pig)
促生長激素釋放激素(GHRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Hormone Releasing Hormone (GHRH)48T/96TY10557Rattus norvegicus (Rat)
促性腺激素釋放激素(GnRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Gonadotropin Releasing Hormone (GnRH)48T/96TY10558Homo sapiens (Human)
催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Prolactin (PRL)48T/96TY10559Rattus norvegicus (Rat)
催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Prolactin (PRL)48T/96TY10560Mus musculus (Mouse)
催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Prolactin (PRL)48T/96TY10561Homo sapiens (Human)
抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Resistin (RETN)48T/96TY10562Homo sapiens (Human)
抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Resistin (RETN)48T/96TY10563Rattus norvegicus (Rat)
抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Resistin (RETN)48T/96TY10564Mus musculus (Mouse)
多巴胺(DA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Dopamine (DA)48T/96TY10565General
蛋白酶激活受體2(PAR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protease Activated Receptor 2 (PAR2)48T/96TY10566Homo sapiens (Human)
胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caspase 8 (CASP8)48T/96TY10567Homo sapiens (Human)
S100鈣結(jié)合蛋白A11(S100A11)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein A11 (S100A11)48T/96TY10568Oryctolagus cuniculus (Rabbit)
S100鈣結(jié)合蛋白B(S100B)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein B (S100B)48T/96TY10569Oryctolagus cuniculus (Rabbit)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 10 (IL10)48T/96TY10570Oryctolagus cuniculus (Rabbit)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY10571Oryctolagus cuniculus (Rabbit)
白介素4(IL4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 4 (IL4)48T/96TY10572Oryctolagus cuniculus (Rabbit)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 6 (IL6)48T/96TY10573Oryctolagus cuniculus (Rabbit)
白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 8 (IL8)48T/96TY10574Oryctolagus cuniculus (Rabbit)
白血病抑制因子受體(LIFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leukemia Inhibitory Factor Receptor (LIFR)48T/96TY10575Oryctolagus cuniculus (Rabbit)
腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epinephrine (EPI)48T/96TY10576General
表皮生長因子受體(EGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epidermal Growth Factor Receptor (EGFR)48T/96TY10577Mus musculus (Mouse)
骨鈣素(OC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Osteocalcin (OC)48T/96TY10578Sus scrofa; Porcine (Pig)
骨鈣素(OC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Osteocalcin (OC)48T/96TY10579Mus musculus (Mouse)
補(bǔ)體成分3(C3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Complement Component 3 (C3)48T/96TY10580Sus scrofa; Porcine (Pig)
單核細(xì)胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY10581Oryctolagus cuniculus (Rabbit)
緩激肽(BK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bradykinin (BK)48T/96TY10582Rattus norvegicus (Rat)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY10583Oryctolagus cuniculus (Rabbit)
肝細(xì)胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY10584Oryctolagus cuniculus (Rabbit)
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。