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小鼠γ干擾素酶免試劑盒,(IFN-γ)ELISA檢測試劑盒,英文名:IFN-γ ELISA Kit,英文縮寫:IFN-γ ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠γ干擾素ELISA檢測試劑盒,IFN-γ 檢測試劑盒,IFN-γ 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠γ干擾素酶免試劑盒,(IFN-γ)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
GATA結合蛋白5(GATA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for GATA Binding Protein 5 (GATA5)48T/96TY10385Homo sapiens (Human)
免疫球蛋白E(IgE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immunoglobulin E (IgE)48T/96TY10386Homo sapiens (Human)
抗P62抗體(AP62A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anti-P62 Antibody (AP62A)48T/96TY10387Homo sapiens (Human)
蛋白激酶抑制因子α(PKIα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Alpha (PKIa)48T/96TY10388Homo sapiens (Human)
甲狀腺球蛋白(TG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thyroglobulin (TG)48T/96TY10389Homo sapiens (Human)
組蛋白H2B(H2B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Histone H2B (H2B)48T/96TY10390Homo sapiens (Human)
抗核糖體P蛋白抗體(ARPA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anti-Ribosomal P Protein Antibody (ARPA)48T/96TY10391Homo sapiens (Human)
蛋白激酶抑制因子β(PKIβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Beta (PKIb)48T/96TY10392Homo sapiens (Human)
TATA框結合蛋白關聯因子1(TAF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for TATA Box Binding Protein Associated Factor 1 (TAF1)48T/96TY10393Homo sapiens (Human)
尾加壓素2(UST2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Urotensin 2 (UST2)48T/96TY10394Homo sapiens (Human)
血小板內皮細胞粘附分子1(PECAM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plaet/Endothelial Cell Adhesion Molecule (PECAM1)48T/96TY10395Homo sapiens (Human)
抗核糖體抗體(Anti-rRNP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anti-Ribosomal RNP Antibody (Anti-rRNP)48T/96TY10396Homo sapiens (Human)
和肽素(CPP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Copeptin (CPP)48T/96TY10397Homo sapiens (Human)
Biopyrrin蛋白(BPn)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Biopyrrin (BPn)48T/96TY10398Homo sapiens (Human)
線粒體肌酸激酶1B(CKMT1B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase, Mitochondrial 1B (CKMT1B)48T/96TY10399Homo sapiens (Human)
普列克底物蛋白同源物樣域家族A成員2(PHLDA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pleckstrin Homology Like Domain Family A, Member 2 (PHLDA2)48T/96TY10400Homo sapiens (Human)
基質金屬蛋白酶12(MMP12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 12 (MMP12)48T/96TY10401Homo sapiens (Human)
肌原纖蛋白2(FBN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibrillin 2 (FBN2)48T/96TY10402Homo sapiens (Human)
粘蛋白17(MUC17)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mucin 17 (MUC17)48T/96TY10403Homo sapiens (Human)
性別決定區(qū)Y框蛋白1(SOX1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sex Determining Region Y Box Protein 1 (SOX1)48T/96TY10404Homo sapiens (Human)
性別決定區(qū)Y框蛋白2(SOX2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sex Determining Region Y Box Protein 2 (SOX2)48T/96TY10405Homo sapiens (Human)
性別決定區(qū)Y框蛋白3(SOX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sex Determining Region Y Box Protein 3 (SOX3)48T/96TY10406Homo sapiens (Human)
激肽釋放酶9(KLK9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 9 (KLK9)48T/96TY10407Homo sapiens (Human)
視網膜鈣黏附蛋白(RCAD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cadherin, Retinal (RCAD)48T/96TY10408Homo sapiens (Human)
GATA結合蛋白3(GATA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for GATA Binding Protein 3 (GATA3)48T/96TY10409Homo sapiens (Human)
鈣非依賴性磷脂酶A2(iPLA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phospholipase A2, Calcium Independent (iPLA2)48T/96TY10410Homo sapiens (Human)
α-血紅蛋白穩(wěn)定蛋白(αHSP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP)48T/96TY10411Homo sapiens (Human)
粘蛋白1(MUC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mucin 1 (MUC1)48T/96TY10412Homo sapiens (Human)
性別決定區(qū)Y框蛋白18(SOX18)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sex Determining Region Y Box Protein 18 (SOX18)48T/96TY10413Homo sapiens (Human)
彈性蛋白微原纖維界面因子1(EMILIN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Elastin Microfibril Interface Located Protein 1 (EMILIN1)48T/96TY10414Homo sapiens (Human)
早期生長應答因子1(EGR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Early Growth Response Protein 1 (EGR1)48T/96TY10415Homo sapiens (Human)
鞘磷脂脂質蛋白1(PLP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Proteolipid Protein 1, Myelin (PLP1)48T/96TY10416Homo sapiens (Human)
髓鞘少突膠質細胞糖蛋白(MOG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myelin Oligodendrocyte Glycoprotein (MOG)48T/96TY10417Homo sapiens (Human)
髓鞘關聯糖蛋白(MAG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myelin Associated Glycoprotein (MAG)48T/96TY10418Homo sapiens (Human)
八聚體結合轉錄因子4(OCT4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4)48T/96TY10419Homo sapiens (Human)
生長分化因子9(GDF9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 9 (GDF9)48T/96TY10420Homo sapiens (Human)
黃體激素(LH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Luteinizing Hormone (LH)48T/96TY10421Homo sapiens (Human)
黃體激素(LH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Luteinizing Hormone (LH)48T/96TY10422Rattus norvegicus (Rat)
黃體激素(LH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Luteinizing Hormone (LH)48T/96TY10423Mus musculus (Mouse)
C-肽(C-Peptide)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for C-Peptide48T/96TY10424Homo sapiens (Human)
C-肽(C-Peptide)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for C-Peptide48T/96TY10425Rattus norvegicus (Rat)
C-肽(C-Peptide)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for C-Peptide48T/96TY10426Mus musculus (Mouse)
胰島素(INS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin (INS)48T/96TY10427Homo sapiens (Human)
胰島素(INS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin (INS)48T/96TY10428Rattus norvegicus (Rat)
激肽釋放酶5(KLK5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 5 (KLK5)48T/96TY10429Homo sapiens (Human)
激肽釋放酶5(KLK5)檢測試劑盒(酶聯免疫吸附試驗法) 小鼠γ干擾素酶免試劑盒,(IFN-γ)ELISA檢測試劑盒ELISA Kit for Kallikrein 5 (KLK5)48T/96TY10430Mus musculus (Mouse)
甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thyroxine (T4)48T/96TY10431General
激肽釋放酶5(KLK5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 5 (KLK5)48T/96TY10432Rattus norvegicus (Rat)
各種類型ELISA測定時,小鼠γ干擾素酶免試劑盒,(IFN-γ)ELISA檢測試劑盒 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。