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人抗環(huán)胍氨酸肽抗體酶免試劑盒,(CCP)ELISA檢測試劑盒,英文名:CCP ELISA Kit,英文縮寫:CCP ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗環(huán)胍氨酸肽抗體ELISA檢測試劑盒,CCP 檢測試劑盒,CCP 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗環(huán)胍氨酸肽抗體酶免試劑盒,(CCP)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
血管生成素1(ANGPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 1 (ANGPT1)48T/96TY08635Sus scrofa; Porcine (Pig)
內(nèi)皮細胞TEK酪氨酸激酶(Tie2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2)48T/96TY08636Sus scrofa; Porcine (Pig)
血管生成素2(ANGPT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 2 (ANGPT2)48T/96TY08637Sus scrofa; Porcine (Pig)
白介素23(IL23)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 23 (IL23)48T/96TY08638Homo sapiens (Human)
白介素23(IL23)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 23 (IL23)48T/96TY08639Rattus norvegicus (Rat)
白介素23(IL23)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 23 (IL23)48T/96TY08640Mus musculus (Mouse)
白介素27(IL27)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 27 (IL27)48T/96TY08641Homo sapiens (Human)
白介素27(IL27)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 27 (IL27)48T/96TY08642Rattus norvegicus (Rat)
白介素27(IL27)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 27 (IL27)48T/96TY08643Mus musculus (Mouse)
多巴胺受體D3(DRD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D3 (DRD3)48T/96TY08644Homo sapiens (Human)
多巴胺受體D3(DRD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D3 (DRD3)48T/96TY08645Rattus norvegicus (Rat)
多巴胺受體D3(DRD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D3 (DRD3)48T/96TY08646Mus musculus (Mouse)
糖原磷酸化酶B(PYGB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Brain (PYGB)48T/96TY08647Homo sapiens (Human)
糖原磷酸化酶B(PYGB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Brain (PYGB)48T/96TY08648Rattus norvegicus (Rat)
糖原磷酸化酶B(PYGB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Brain (PYGB)48T/96TY08649Mus musculus (Mouse)
糖原磷酸化酶M(PYGM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Muscle (PYGM)48T/96TY08650Homo sapiens (Human)
糖原磷酸化酶M(PYGM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Muscle (PYGM)48T/96TY08651Rattus norvegicus (Rat)
糖原磷酸化酶M(PYGM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Muscle (PYGM)48T/96TY08652Mus musculus (Mouse)
糖原磷酸化酶L(PYGL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Liver (PYGL)48T/96TY08653Homo sapiens (Human)
糖原磷酸化酶L(PYGL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Liver (PYGL)48T/96TY08654Rattus norvegicus (Rat)
糖原磷酸化酶L(PYGL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Phosphorylase, Liver (PYGL)48T/96TY08655Mus musculus (Mouse)
胃泌素抑制肽(GIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gastric Inhibitory Polypeptide (GIP)48T/96TY08656Homo sapiens (Human)
胃泌素抑制肽(GIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gastric Inhibitory Polypeptide (GIP)48T/96TY08657Rattus norvegicus (Rat)
胃泌素抑制肽(GIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gastric Inhibitory Polypeptide (GIP)48T/96TY08658Mus musculus (Mouse)
脂蛋白脂酶(LIPD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Lipoprotein (LIPD)48T/96TY08659Rattus norvegicus (Rat)
脂蛋白脂酶(LIPD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Lipoprotein (LIPD)48T/96TY08660Mus musculus (Mouse)
抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA)48T/96TY08661Homo sapiens (Human)
抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA)48T/96TY08662Rattus norvegicus (Rat)
抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA)48T/96TY08663Mus musculus (Mouse)
肌原纖蛋白1(FBN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrillin 1 (FBN1)48T/96TY08664Homo sapiens (Human)
肌原纖蛋白1(FBN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrillin 1 (FBN1)48T/96TY08665Rattus norvegicus (Rat)
肌原纖蛋白1(FBN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrillin 1 (FBN1)48T/96TY08666Mus musculus (Mouse)
E選擇素(SELE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selectin, Endothelium (SELE)48T/96TY08667Rattus norvegicus (Rat)
卵泡抑素(FS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Follistatin (FS)48T/96TY08668Homo sapiens (Human)
卵泡抑素(FS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Follistatin (FS)48T/96TY08669Rattus norvegicus (Rat)
卵泡抑素(FS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Follistatin (FS)48T/96TY08670Mus musculus (Mouse)
補體3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 3a (C3a)48T/96TY08671Homo sapiens (Human)
補體3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 3a (C3a)48T/96TY08672Rattus norvegicus (Rat)
補體3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 3a (C3a)48T/96TY08673Mus musculus (Mouse)
補體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 5a (C5a)48T/96TY08674Homo sapiens (Human)
補體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 5a (C5a)48T/96TY08675Rattus norvegicus (Rat)
補體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 5a (C5a)48T/96TY08676Mus musculus (Mouse)
C4結(jié)合蛋白α(C4BPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C4 Binding Protein Alpha (C4BPa)48T/96TY08677Homo sapiens (Human)
C4結(jié)合蛋白α(C4BPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C4 Binding Protein Alpha (C4BPa)48T/96TY08678Rattus norvegicus (Rat)
C4結(jié)合蛋白α(C4BPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C4 Binding Protein Alpha (C4BPa)48T/96TY08679Mus musculus (Mouse)
二肽基肽酶Ⅳ(DPP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dipeptidyl Peptidase IV (DPP4)48T/96TY08680Homo sapiens (Human)
二肽基肽酶Ⅳ(DPP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dipeptidyl Peptidase IV (DPP4)48T/96TY08681Rattus norvegicus (Rat)
二肽基肽酶Ⅳ(DPP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dipeptidyl Peptidase IV (DPP4)48T/96TY08682Mus musculus (Mouse)
蛋白激酶抑制因子γ(PKIγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Gamma (PKIg)48T/96TY08683Homo sapiens (Human)
蛋白激酶抑制因子γ(PKIγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Gamma (PKIg)48T/96TY08684Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。